Jon Glaser (born June 20, 1968 in Chicago, Illinois) is a writer and actor based out of New York City. He has written for and appeared on Late Night with Conan O'Brien and appeared in guest-starring roles on Wonder Showzen and Aqua Teen Hunger Force. He had a role in the 2006 movie, School for Scoundrels and supplied voices for the show, Freak Show. Currently, he voices DJ Jesus in Lucy the Daughter of The Devil.

Glaser was a member of the mainstage cast of The Second City during the mid-1990s, performing alongside future Saturday Night Live head writer Adam McKay and cast member Rachel Dratch, as well as future Mr. Show and 30 Rock cast member Scott Adsit. In Second City's award-winning revue Pinata Full of Bees, which was directed by Tom Gianas, Glaser sang about the importance of not betraying a friendship by pretending not to have legs in order to play in a wheelchair basketball league. He also provided musical accompaniment for the show's climax by drumming in a demonic pig mask, and appeared onstage throughout the show to pass judgement on audience members for laughing at jokes he considered socially irresponsible.

After leaving Second City, Glaser performed on Jenny McCarthy's short-lived sketch comedy series, and also made one appearance as a correspondent on The Daily Show. Currently, he performs with the New York City-based band A Matter of Trust. They performed with The New Pornographers in 2006. He maintains a comedic partnership with H. Jon Benjamin as the Midnight Pajama Jam, and he also stars in and writes the online Comedy Central show 'Tiny Hands'. Glaser starred in the short-lived cartoon Stroker & Hoop and is a frequent guest on the Human Giant's MTV sketch comedy show.

Alumnus of Comedy Company at the University of Michigan.